Formats of InnovaDx ® OneStep kits:
Str
Lyophilized ready-to-use reaction mixture in white strips in a 96-well plate (8 x 0.2 ml tubes), to which extracted DNA/RNA samples are added.
Uni
Lyophilized ready-to-use reaction mixture in clear 0.2 ml tubes, to which extracted DNA/RNA is added, one tube equals one reaction.
- All necessary components for the PCR reaction are part of the lyophilized reaction mixture. In the case of PCR with reverse transcription (RT-PCR), a separate reverse transcription step is not required.
- To set up the PCR reaction, it is sufficient to only add samples of extracted DNA/RNA and initiate PCR or RT-PCR.
Real-Time PCR instrument compatibility with formats of InnovaDx ® OneStep kits:
Str
CFX96 (Bio-Rad, USA),
DTprime (DT-96), DTlite (DNA Technology, Russia),
Gentier 96E/R (Xi’an TianLong, Science and Technology Co., Ltd., China).
Uni
CFX96 (Bio-Rad, USA),
DTprime (DT-96), DTlite (DNA Technology, Russia),
Rotor-Gene Q (Qiagen, Germany),
Rotor-Gene 3000/6000 (Corbett Research, Australia),
Gentier 96E/R (Xi’an TianLong, Science and Technology Co., Ltd., China).
| NUMBER OF SIMULTANEOUSLY DETECTABLE PATHOGENS | DETECTION CHANNELS |
|---|---|
| 1 | FAM, ROX |
| 2 | FAM, ROX, HEX (JOE) |
| 3 and more | FAM, ROX, HEX (JOE), Cy5 |
Other Real-Time PCR instruments with similar technical characteristics can be used after validation.
InnovaDx ® TwoStep kits format :
Lyophilized Master Mix in 0.2 ml 8-tube strips, add diluent and extracted DNA/RNA into the tubes.
Real-time PCR instrument compatibility with InnovaDx ® TwoStep kits :
CFX96™ (Bio-Rad, USA) or other Real-Time PCR systems with FAM, R6G, ROX, Cy5 channels (or their spectral analogues) with similar spectral characteristics.